Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

The production of recombinant membrane proteins for structural and functional studies remains technically challenging due to low levels of expression and the inherent instability of many membrane proteins once solubilized in detergents. A protocol is described that combines ligation independent clon...

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Publicat a:J Vis Exp
Autors principals: Bird, Louise E., Rada, Heather, Verma, Anil, Gasper, Raphael, Birch, James, Jennions, Matthew, Lӧwe, Jan, Moraes, Isabel, Owens, Raymond J.
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2015
Matèries:
Microbiology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354503/
https://ncbi.nlm.nih.gov/pubmed/25590335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52357
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