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Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
The production of recombinant membrane proteins for structural and functional studies remains technically challenging due to low levels of expression and the inherent instability of many membrane proteins once solubilized in detergents. A protocol is described that combines ligation independent clon...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4354503/ https://ncbi.nlm.nih.gov/pubmed/25590335 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52357 |
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