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Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

The accessibility of high-throughput sequencing has revolutionized many fields of biology. In order to better understand host-associated viral and microbial communities, a comprehensive workflow for DNA and RNA extraction was developed. The workflow concurrently generates viral and microbial metagen...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Lim, Yan Wei, Haynes, Matthew, Furlan, Mike, Robertson, Charles E., Harris, J. Kirk, Rohwer, Forest
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354477/
https://ncbi.nlm.nih.gov/pubmed/25549184
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52117
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