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Evaluation of a Homemade SYBR(®) Green I Reaction Mixture for Real-Time PCR Quantification of Gene Expression
Real-time PCR is an accurate method that can be used for the quantification of specific DNA molecules. Here we provide a protocol for SYBR(®) Green I in real-time PCR applications using plastic reaction tubes. We report that SYBR Green I is alkali labile and once degraded inhibits the PCR. In our op...
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| Publicado no: | Biotechniques |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4353838/ https://ncbi.nlm.nih.gov/pubmed/11962601 |
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