Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits

Purpose: Mouse IgG subclasses containing IgG1, IgG2a, IgG2b and IgG3 have been defined and described both physiochemically and immunologically. Methods: Sepharose beads conjugated with protein A affinity chromatography was used for purification of mouse IgG2b. Sodium citrate buffer (0.1 M, pH: 3.5)...

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Udgivet i:Adv Pharm Bull
Main Authors: Eivazi, Sadeq, Majidi, Jafar, Aghebati Maleki, leili, Abdolalizadeh, Jalal, Yousefi, Mehdi, Ahmadi, Majid, Dadashi, Somayeh, Moradi, Zahra, Zolali, Elmira
Format: Artigo
Sprog:Inglês
Udgivet: Tabriz University of Medical Sciences 2015
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4352212/
https://ncbi.nlm.nih.gov/pubmed/25789227
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5681/apb.2015.015
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