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Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish

The CRISPR/Cas9 system provides a powerful tool for genome editing in various model organisms, including zebrafish. The establishment of targeted gene-disrupted zebrafish (knockouts) is readily achieved by CRISPR/Cas9-mediated genome modification. Recently, exogenous DNA integration into the zebrafi...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Hisano, Yu, Sakuma, Tetsushi, Nakade, Shota, Ohga, Rie, Ota, Satoshi, Okamoto, Hitoshi, Yamamoto, Takashi, Kawahara, Atsuo
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4350073/
https://ncbi.nlm.nih.gov/pubmed/25740433
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep08841
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