Indirect protein quantification of drug-transforming enzymes using peptide group-specific immunoaffinity enrichment and mass spectrometry

Immunoaffinity enrichment of proteotypic peptides, coupled with selected reaction monitoring, enables indirect protein quantification. However the lack of suitable antibodies limits its widespread application. We developed a method in which multi-specific antibodies are used to enrich groups of pept...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Weiß, Frederik, Schnabel, Anke, Planatscher, Hannes, van den Berg, Bart H. J., Serschnitzki, Bettina, Nuessler, Andreas K., Thasler, Wolfgang E., Weiss, Thomas S., Reuss, Matthias, Stoll, Dieter, Templin, Markus F., Joos, Thomas O., Marcus, Katrin, Poetz, Oliver
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4348618/
https://ncbi.nlm.nih.gov/pubmed/25737130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep08759
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