Palladium-based Mass-Tag Cell Barcoding with a Doublet-Filtering Scheme and Single Cell Deconvolution Algorithm

Mass-tag cell barcoding (MCB) labels individual cell samples with unique combinatorial barcodes, after which they are pooled for processing and measurement as a single multiplexed sample. The MCB method eliminates variability between samples in antibody staining and instrument sensitivity, reduces a...

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發表在:Nat Protoc
Main Authors: Zunder, Eli R., Finck, Rachel, Behbehani, Gregory K., Amir, El-ad D., Krishnaswamy, Smita, Gonzalez, Veronica D., Lorang, Cynthia G., Bjornson, Zach, Spitzer, Matthew H., Bodenmiller, Bernd, Fantl, Wendy J., Pe’er, Dana, Nolan, Garry P.
格式: Artigo
語言:Inglês
出版: 2015
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4347881/
https://ncbi.nlm.nih.gov/pubmed/25612231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.020
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