Spotting effect in microarray experiments

BACKGROUND: Microarray data must be normalized because they suffer from multiple biases. We have identified a source of spatial experimental variability that significantly affects data obtained with Cy3/Cy5 spotted glass arrays. It yields a periodic pattern altering both signal (Cy3/Cy5 ratio) and i...

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Bibliografiska uppgifter
Huvudupphovsmän: Mary-Huard, Tristan, Daudin, Jean-Jacques, Robin, Stéphane, Bitton, Frédérique, Cabannes, Eric, Hilson, Pierre
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2004
Ämnen:
Methodology Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC434492/
https://ncbi.nlm.nih.gov/pubmed/15151695
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-5-63
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