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Rapid preparation of RNA samples for NMR spectroscopy and X-ray crystallography

Knowledge of the three-dimensional structures of RNA and its complexes is important for understanding the molecular mechanism of RNA recognition by proteins or ligands. Enzymatic synthesis using T7 bacteriophage RNA polymerase is used to prepare samples for NMR spectroscopy and X-ray crystallography...

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Detalhes bibliográficos
Main Authors: Cheong, Hae-Kap, Hwang, Eunha, Lee, Chulhyun, Choi, Byong-Seok, Cheong, Chaejoon
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC434460/
https://ncbi.nlm.nih.gov/pubmed/15199176
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh081
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