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Rapid preparation of RNA samples for NMR spectroscopy and X-ray crystallography

Knowledge of the three-dimensional structures of RNA and its complexes is important for understanding the molecular mechanism of RNA recognition by proteins or ligands. Enzymatic synthesis using T7 bacteriophage RNA polymerase is used to prepare samples for NMR spectroscopy and X-ray crystallography...

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Autores principales: Cheong, Hae-Kap, Hwang, Eunha, Lee, Chulhyun, Choi, Byong-Seok, Cheong, Chaejoon
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2004
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC434460/
https://ncbi.nlm.nih.gov/pubmed/15199176
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh081
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