Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy

Fluorescent proteins facilitate a variety of imaging paradigms in live and fixed samples. However, they cease to function following heavy fixation, hindering advanced applications such as correlative light and electron microscopy. Here we report engineered variants of the photoconvertible Eos fluore...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Paez Segala, Maria G., Sun, Mei G., Shtengel, Gleb, Viswanathan, Sarada, Baird, Michelle A., Macklin, John J., Patel, Ronak, Allen, John R., Howe, Elizabeth S., Piszczek, Grzegorz, Hess, Harald F., Davidson, Michael W., Wang, Yalin, Looger, Loren L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4344411/
https://ncbi.nlm.nih.gov/pubmed/25581799
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3225
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