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Photo-Affinity Labeling of tRNA Binding Sites in Macromolecules. I. Linking of the Phenacyl-p-azide of 4-Thiouridine in (Escherichia coli) Valyl-tRNA to 16S RNA at the Ribosomal P Site

The phenacyl-p-azide of 4-thiouridine in (E. coli) tRNA(1)(Val) was prepared for use as a photo-affinity probe of tRNA binding sites on ribosomes. The derivatized tRNA was 90-100% as active as control tRNA for aminoacylation, nonenzymatic binding to the ribosomal P site, elongation factor Tu(EFTu)-d...

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Detalhes bibliográficos
Main Authors: Schwartz, Ira, Ofengand, James
Formato: Artigo
Idioma:Inglês
Publicado em: 1974
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC434304/
https://ncbi.nlm.nih.gov/pubmed/4610566
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