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Diagonal Polyacrylamide-Dodecyl Sulfate Gel Electrophoresis for the Identification of Ribosomal Proteins Crosslinked with Methyl-4-Mercaptobutyrimidate

A diagonal polyacrylamide-dodecyl sulfate gel electrophoresis procedure is described. Its utility is related to the use of the reagent methyl-4-mercaptobutyrimidate as a protein-protein crosslinking reagent. Crosslinking with this reagent occurs through the formation of intermolecular disulfide bond...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Principais autores: Sommer, Andreas, Traut, Robert R.
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 1974
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC434303/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4610565/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.10.3946
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