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SSB–DNA Binding Monitored by Fluorescence Intensity and Anisotropy
Fluorescence methods have proven to be extremely useful tools for quantitative studies of the equilibria and kinetics of protein–DNA interactions. If the protein contains tryptophan (Trp), as is often the case, and there is a change in intrinsic Trp fluorescence of the protein, one can use this chan...
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| 出版年: | Methods Mol Biol |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2012
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4342854/ https://ncbi.nlm.nih.gov/pubmed/22976177 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-032-8_4 |
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