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SSB–DNA Binding Monitored by Fluorescence Intensity and Anisotropy
Fluorescence methods have proven to be extremely useful tools for quantitative studies of the equilibria and kinetics of protein–DNA interactions. If the protein contains tryptophan (Trp), as is often the case, and there is a change in intrinsic Trp fluorescence of the protein, one can use this chan...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4342854/ https://ncbi.nlm.nih.gov/pubmed/22976177 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-032-8_4 |
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