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Purification and Characterization of Recombinant Staphylococcus haemolyticus DNA Gyrase and Topoisomerase IV Expressed in Escherichia coli

The subunits of DNA gyrase and topoisomerase IV from Staphylococcus haemolyticus were expressed in Escherichia coli, purified to homogeneity, and used to reconstitute active enzymes that were sensitive to known topoisomerase inhibitors. This represents the first description of a method for isolating...

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Autori principali: Bronstein, Joel C., Olson, Stacey L., LeVier, Kristin, Tomilo, Mark, Weber, Peter C.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2004
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC434177/
https://ncbi.nlm.nih.gov/pubmed/15215131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.48.7.2708-2711.2004
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