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Bromodeoxyuridine-Labeled Viral Particles as a Tool for Visualization of the Immediate-Early Events of Human Cytomegalovirus Infection

We describe here a simple method for labeling the genome of human cytomegalovirus, a large double-stranded DNA virus, with bromodeoxyuridine (BrdU). The labeled DNA was incorporated into viral particles, which were then collected in cell supernatant. To demonstrate the versatility and effectiveness...

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Detalhes bibliográficos
Main Authors: Rosenke, Kyle, Fortunato, Elizabeth A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC434117/
https://ncbi.nlm.nih.gov/pubmed/15220456
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.78.14.7818-7822.2004
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