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Human Coronavirus 229E Nonstructural Protein 13: Characterization of Duplex-Unwinding, Nucleoside Triphosphatase, and RNA 5′-Triphosphatase Activities

The human coronavirus 229E (HCoV-229E) replicase gene-encoded nonstructural protein 13 (nsp13) contains an N-terminal zinc-binding domain and a C-terminal superfamily 1 helicase domain. A histidine-tagged form of nsp13, which was expressed in insect cells and purified, is reported to unwind efficien...

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Detalhes bibliográficos
Main Authors: Ivanov, Konstantin A., Ziebuhr, John
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC434081/
https://ncbi.nlm.nih.gov/pubmed/15220459
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.78.14.7833-7838.2004
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