Identification of 5-Bromo-2’-Deoxyuridine-Labeled Cells during Mouse Spermatogenesis by Heat-Induced Antigen Retrieval in Lectin Staining and Immunohistochemistry

DNA replication occurs during S-phase in spermatogonia and preleptotene spermatocytes during spermatogenesis. 5-Bromo-2’-deoxyuridine (BrdU) is incorporated into synthesized DNA and is detectable in the nucleus by immunohistochemistry (IHC). To identify BrdU-labeled spermatogenic cells, the spermato...

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Veröffentlicht in:J Histochem Cytochem
Hauptverfasser: Wakayama, Tomohiko, Nakata, Hiroki, Kumchantuek, Tewarat, Gewaily, Mahmoud Saad, Iseki, Shoichi
Format: Artigo
Sprache:Inglês
Veröffentlicht: SAGE Publications 2015
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Articles
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4340735/
https://ncbi.nlm.nih.gov/pubmed/25479790
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/0022155414564870
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