Improved Protein Extractionand Protein Identification from Archival Formalin-fixed Paraffin-embedded Human Aortas

PURPOSE: Evaluate combination of heat and elevated pressure to enhance protein extraction and quality of formaldehyde-fixed (FF), and FF paraffin-embedded (FFPE) aorta for proteomics. EXPERIMENT DESIGN: Proteins were extracted from fresh frozen aorta at RT. FF and FFPE aortas (3 months and 15 years)...

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Publicado no:Proteomics Clin Appl
Main Authors: Fu, Zongming, Yan, Kun, Rosenberg, Avraham, Jin, Zhicheng, Crain, Barbara, Athas, Grace, Vander Heide, Richard S, Howard, Timothy, Everett, Allen D., Herrington, David, Van Eyk, Jennifer E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4340701/
https://ncbi.nlm.nih.gov/pubmed/23339088
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prca.201200064
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