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The recBC Deoxyribonuclease of Escherichia coli: Isolation and Characterization of the Subunit Proteins and Reconstitution of the Enzyme
After dissociation of the E. coli recBc DNase (ATP-dependent DNase) with concentrated NaCl, two subunit proteins were isolated by ion exchange chromatography. Combination and subsequent incubation of the subunits resulted in the appearance of the original DNase. The subunit proteins, designated α an...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1974
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433988/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4280072/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.12.4816 |
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