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Translational Properties of Rabbit Globin mRNA after Specific Removal of Poly(A) with Ribonuclease H

Highly purified RNase H (RNA·DNA hybrid ribonucleotidohydrolase, EC 3.1.4.34) from calf thymus was used to specifically remove the poly(A) sequences of purified rabbit globin mRNA after its hybridization with poly(dT). The deadenylylated globin mRNA was repurified by a one-step procedure including a...

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Detalles Bibliográficos
Main Authors: Sippel, A. E., Stavrianopoulos, J. G., Schutz, G., Feigelson, P.
Formato: Artigo
Idioma:Inglês
Publicado: 1974
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC433943/
https://ncbi.nlm.nih.gov/pubmed/4531006
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