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Packaging and Maturation of DNA of Bacteriophage T7 In Vitro

We have developed an in vitro complementation assay to demonstrate packaging and maturation of DNA of phage T7. Cells of Escherichia coli B infected with an appropriate T7 amber mutant are concentrated 200-fold and lysed by freezing and thawing. Two extracts from cells infected with different amber...

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Detalhes bibliográficos
Main Authors: Kerr, Carol, Sadowski, Paul D.
Formato: Artigo
Idioma:Inglês
Publicado em: 1974
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC433811/
https://ncbi.nlm.nih.gov/pubmed/4530321
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