Lattice Light Sheet Microscopy: Imaging Molecules to Embryos at High Spatiotemporal Resolution

Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, too small, or occur too rapidly to see clearly with existing tools. We crafted ultra-thin light sheets from two-dimensional optical lattices that allowed us...

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Published in:Science
Main Authors: Chen, Bi-Chang, Legant, Wesley R., Wang, Kai, Shao, Lin, Milkie, Daniel E., Davidson, Michael W., Janetopoulos, Chris, Wu, Xufeng S., Hammer, John A., Liu, Zhe, English, Brian P., Mimori-Kiyosue, Yuko, Romero, Daniel P., Ritter, Alex T., Lippincott-Schwartz, Jennifer, Fritz-Laylin, Lillian, Mullins, R. Dyche, Mitchell, Diana M., Bembenek, Joshua N., Reymann, Anne-Cecile, Böhme, Ralph, Grill, Stephan W., Wang, Jennifer T., Seydoux, Geraldine, Tulu, U. Serdar, Kiehart, Daniel P., Betzig, Eric
Format: Artigo
Language:Inglês
Published: 2014
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4336192/
https://ncbi.nlm.nih.gov/pubmed/25342811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1257998
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