A New Enzymatic Assay for Guanosine 3′:5′-Cyclic Monophosphate and Its Application to the Ductus Deferens of the Rat

A sensitive enzymatic procedure has been developed for the determination of guanosine 3′:5′-cyclic monophosphate (cyclic GMP). It is based on the conversion of cyclic GMP to GMP by cyclic nucleotide phosphodiesterase and on the transfer of (32)P from [γ-(32)P]ATP to GMP by the action of a specific A...

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Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Schultz, G., Hardman, J. G., Schultz, K., Davis, J. W., Sutherland, E. W.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1973
Fag:
Biological Sciences: Biochemistry
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433581/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4352651/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.6.1721
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