Simple regression for correcting ΔC(t) bias in RT-qPCR low-density array data normalization

BACKGROUND: Reverse transcription quantitative PCR (RT-qPCR) is considered the gold standard for quantifying relative gene expression. Normalization of RT-qPCR data is commonly achieved by subtracting the C(t) values of the internal reference genes from the C(t) values of the target genes to obtain...

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Detalhes bibliográficos
Publicado no:BMC Genomics
Main Authors: Cui, Xiangqin, Yu, Shaohua, Tamhane, Ashutosh, Causey, Zenoria L, Steg, Adam, Danila, Maria I, Reynolds, Richard J, Wang, Jinyi, Wanzeck, Keith C, Tang, Qi, Ledbetter, Stephanie S, Redden, David T, Johnson, Martin R, Bridges, S Louis
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4335788/
https://ncbi.nlm.nih.gov/pubmed/25888492
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-015-1274-1
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