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An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination

A better understanding of the immune responses of chickens to the influenza virus is essential for the development of new strategies of vaccination and control. We have developed a method incorporating infected chicken kidney cells (CKC) in culture with splenocytes in an IFNγ ELISpot assay to enumer...

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Detalhes bibliográficos
Publicado no:J Immunol Methods
Main Authors: Ruiz-Hernandez, Raul, Peroval, Marylene, Boyd, Amy, Balkissoon, Devanand, Staines, Karen, Smith, Adrian, Butter, Colin
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4334094/
https://ncbi.nlm.nih.gov/pubmed/25450002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2014.10.012
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