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Yeast manno-protein biosynthesis: solubilization and selective assay of four mannosyltransferases.
Using appropriate yeast strains and exogenous acceptors, we have devised specific assays for four mannosyltransferase activities involved in biosynthesis of the carbohydrate outer chain of yeast mannoproteins. The assays utilize GDP-[14C]mannose as the donor and unlabeled oligosaccharides as the acc...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1975
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433106/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1105568/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.72.10.3912 |
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