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phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli.

Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. The reaction is rifampicin-resistant, requires single-stranded DNA, Mg++, deoxynucleoside triphosphate...

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Main Authors: Schuster, H, Mikolajczyk, M, Rohrschneider, J, Geschke, B
格式: Artigo
語言:Inglês
出版: 1975
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC433105/
https://ncbi.nlm.nih.gov/pubmed/670
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