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SILAC-iPAC: A quantitative method for distinguishing genuine from non-specific components of protein complexes by parallel affinity capture
Pull-down assays can identify members of protein complexes but suffer from co-isolation of contaminants. The problem is particularly acute when the specifically interacting partners are of low-abundance and/or bind transiently with low affinity. To differentiate true interacting partners from contam...
Αποθηκεύτηκε σε:
Τόπος έκδοσης: | J Proteomics |
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Κύριοι συγγραφείς: | , , |
Μορφή: | Artigo |
Γλώσσα: | Inglês |
Έκδοση: |
Elsevier
2015
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Θέματα: | |
Διαθέσιμο Online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4329988/ https://ncbi.nlm.nih.gov/pubmed/25534881 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jprot.2014.12.006 |
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