Sequence-specific "gene signatures" can be obtained by PCR with single specific primers at low stringency.

Low-stringency single specific primer PCR (LSSP-PCR) is an extremely simple PCR-based technique that detects single or multiple mutations in gene-sized DNA fragments. A purified DNA fragment is subjected to PCR using high concentrations of a single specific oligonucleotide primer, large amounts of T...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Pena, S D, Barreto, G, Vago, A R, De Marco, L, Reinach, F C, Dias Neto, E, Simpson, A J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1994
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC43281/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8127912/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.5.1946
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