Sequence-specific "gene signatures" can be obtained by PCR with single specific primers at low stringency.

Low-stringency single specific primer PCR (LSSP-PCR) is an extremely simple PCR-based technique that detects single or multiple mutations in gene-sized DNA fragments. A purified DNA fragment is subjected to PCR using high concentrations of a single specific oligonucleotide primer, large amounts of T...

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Detalles Bibliográficos
Autores principales: Pena, S D, Barreto, G, Vago, A R, De Marco, L, Reinach, F C, Dias Neto, E, Simpson, A J
Formato: Artigo
Lenguaje:Inglês
Publicado: 1994
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC43281/
https://ncbi.nlm.nih.gov/pubmed/8127912
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