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Excision of prophage lambda in a cell-free system.

A cell-free system that promotes the excision of prophage lambda DNA has been established. The substrate for the reaction is phage DNA carrying two attachment sites, which, in vivo, undergoes intramolecular recombination between these sites. The in vitro recombination system is efficient; 25-35% of...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Gottesman, S, Gottesman, M
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1975
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC432722/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1094457/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.72.6.2188
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