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Role of nucleotides in tubulin polymerization: effect of guanylyl 5'-methylenediphosphonate.

Incubation of 48,000 X g rat brain supernatants for 30 min at 37 degrees with 1-2 mM guanylyl 5'-methylenediphosphonate [Gmp(CH2)pp] results in polymerization of 95-98% of the tubulin present. This is considerably more than the 50% polymerization that can be achieved with the natural nucleotide...

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Detalhes bibliográficos
Main Authors: Sandoval, I V, MacDonald, E, Jameson, J L, Cuatrecasas, P
Formato: Artigo
Idioma:Inglês
Publicado em: 1977
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC432060/
https://ncbi.nlm.nih.gov/pubmed/200938
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