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Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation

BACKGROUND: Tissue fixation is crucial for preserving the morphology of biological structures and cytological details to prevent postmortem degradation and autolysis. Improper fixation conditions could lead to artifacts and thus incorrect conclusions in immunofluorescence or histology experiments. T...

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Detalhes bibliográficos
Publicado no:BMC Dev Biol
Main Authors: Nie, Jing, Mahato, Simpla, Zelhof, Andrew C
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4320506/
https://ncbi.nlm.nih.gov/pubmed/25645690
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12861-015-0056-y
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