Ultrasensitive retrovirus detection by a reverse transcriptase assay based on product enhancement.
Reverse transcriptase (RT) is an indispensable component of infectious retroviruses. We have developed an ultrasensitive RT test in which RNA of bacteriophage MS2 serves as the template for RT-mediated cDNA synthesis. A fragment of the cDNA is selectively amplified by polymerase chain reaction and t...
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1994
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC43196/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7509077/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.4.1544 |
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