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Choleragen activation of solubilized adenylate cyclase: Requirement for GTP and protein activator for demonstration of enzymatic activity

The requirements for choleragen activation of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] were investigated by using an enzyme preparation solubilized with Triton X-100 from an extensively washed brain particulate fraction and partially purified with DEAE-cellulose. Unlike th...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Moss, Joel, Vaughan, Martha
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1977
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431949/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/200916/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.10.4396
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