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Studies on the mechanism of phosphorylation of synthetic polypeptides by a calf thymus cyclic AMP-dependent protein kinase

Synthetic polypeptides were employed as substrates in kinetic analyses of the reaction mechanism for the catalytic subunit of a cyclic AMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) from calf thymus. This enzyme preparation was shown to catalyze the transfer of phosphate...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Pomerantz, Arthur H., Allfrey, Vincent G., Merrifield, R. B., Johnson, Edward M.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1977
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431919/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/200911/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.10.4261
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