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Resonance coherent anti-Stokes Raman scattering spectra of fluorescent biological chromophores: Vibrational evidence for hydrogen bonding of flavin to glucose oxidase and for rapid solvent exchange

Coherent anti-Stokes Raman scattering (CARS) spectra are reported for flavin adenine dinucleotide (FAD) and glucose oxidase (β-D-glucose:oxygen 1-oxidoreductase; EC 1.1.3.4), in resonance with the 450 nm flavin absorption band. Several isoalloxazine ring modes were observed (1635, 1584, 1507, 1416,...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Dutta, Prabir K., Nestor, James R., Spiro, Thomas G.
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1977
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431893/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16592440/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.10.4146
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