General Strategy for the Bioorthogonal Incorporation of Strongly Absorbing, Solvation-Sensitive Infrared Probes into Proteins

[Image: see text] A high-sensitivity metal-carbonyl-based IR probe is described that can be incorporated into proteins or other biomolecules in very high yield via Click chemistry. A two-step strategy is demonstrated. First, a methionine auxotroph is used to incorporate the unnatural amino acid azid...

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Detalhes bibliográficos
Publicado no:J Phys Chem B
Main Authors: Peran, Ivan, Oudenhoven, Tracey, Woys, Ann Marie, Watson, Matthew D., Zhang, Tianqi O., Carrico, Isaac, Zanni, Martin T., Raleigh, Daniel P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2014
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4317048/
https://ncbi.nlm.nih.gov/pubmed/24749542
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jp5008279
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