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Production of a functional eukaryotic enzyme in Escherichia coli: cloning and expression of the yeast structural gene for imidazole-glycerolphosphate dehydratase (his3).
A cloned segment of yeast DNA containing the structural gene for imidazoleglycerolphosphate dehydratase (D-erythro-imidazoleglycerolphosphate hydro-lase, EC 4.2.1.19) is transcribed and translated in Escherichia coli with sufficient fidelity to produce functional enzyme. This segment of yeast DNA wa...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1977
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431671/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/341150/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.12.5255 |
| Oznake: |
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