Evaluation of the Performances of Ribosomal Database Project (RDP) Classifier for Taxonomic Assignment of 16S rRNA Metabarcoding Sequences Generated from Illumina-Solexa NGS

Here we report a benchmark of the effect of bootstrap cut-off values of the RDP Classifier tool in terms of data retention along the different taxonomic ranks by using Illumina reads. Results provide guidelines for planning sequencing depths and selection of bootstrap cut-off in taxonomic assignment...

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Detalhes bibliográficos
Publicado no:J Genomics
Main Authors: Bacci, Giovanni, Bani, Alessia, Bazzicalupo, Marco, Ceccherini, Maria Teresa, Galardini, Marco, Nannipieri, Paolo, Pietramellara, Giacomo, Mengoni, Alessio
Formato: Artigo
Idioma:Inglês
Publicado em: Ivyspring International Publisher 2015
Assuntos:
Short Research Communication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4316179/
https://ncbi.nlm.nih.gov/pubmed/25653722
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7150/jgen.9204
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