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Large-scale automated isolation of Escherichia coli mutants with thermosensitive DNA replication.

We have screened about 1.4 million colonies of Escherichia coli K-12 for their ability to grow on nutrient agar at 30 degrees and 41 degrees. Among the 2266 temperature-sensitive mutants found, 110 were defective in DNA synthesis but not in protein synthesis at 41 degrees. Three of these dna mutatio...

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Main Authors: Sevastopoulos, C G, Wehr, C T, Glaser, D A
格式: Artigo
語言:Inglês
出版: 1977
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC431615/
https://ncbi.nlm.nih.gov/pubmed/333440
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