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Binding of synthetic lactose operator DNAs to lactose repressors

The nitrocellulose filter assay was used to study the interactions of wild-type (SQ) and tight-binding (QX86) lac repressors with synthetic lac operators 21 and 26 base pairs long. The repressor binding properties of both operators were very similar, indicating that both contain the same specific re...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Goeddel, D. V., Yansura, D. G., Caruthers, M. H.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1977
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Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431535/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/333432/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.8.3292
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