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Development and Optimization of a Real-Time PCR Assay for Detection of Herpes Simplex and Varicella-Zoster Viruses in Skin and Mucosal Lesions by Use of the BD Max Open System

We transitioned laboratory-developed PCR assays for herpes simplex virus 1 (HSV-1), HSV-2, and varicella-zoster virus (VZV) to the BD Max system by using BD Max open system reagents. After optimization, the agreement with the reference PCR assay was 100% (123/123) for HSV-1, 96.7% (119/123) for HSV-...

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Détails bibliographiques
Publié dans:J Clin Microbiol
Auteurs principaux: Cárdenas, Ana María, Edelstein, Paul H., Alby, Kevin
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4313320/
https://ncbi.nlm.nih.gov/pubmed/25275002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02237-14
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