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Evaluation of Five Real-Time PCR Assays for Detection of Mycoplasma pneumoniae

Four commercial real-time PCR assays to detect Mycoplasma pneumoniae were tested, and the results were compared with the results for an in-house approach. Despite differences of crossing threshold values of up to 4, assays were able to detect at least 20 CFU/5 μl (52 fg DNA/5 μl) of sample with the...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Dumke, Roger, Jacobs, Enno
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4313229/
https://ncbi.nlm.nih.gov/pubmed/25210063
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02048-14
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