Application of Coamplification at Lower Denaturation Temperature-PCR Sequencing for Early Detection of Antiviral Drug Resistance Mutations of Hepatitis B Virus

Nucleoside/nucleotide analogue for the treatment of chronic hepatitis B virus (HBV) infection is hampered by the emergence of drug resistance mutations. Conventional PCR sequencing cannot detect minor variants of <20%. We developed a modified co-amplification at lower denaturation temperature-PCR...

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Enregistré dans:
Détails bibliographiques
Publié dans:J Clin Microbiol
Auteurs principaux: Wong, Danny Ka-Ho, Tsoi, Ottilia, Huang, Fung-Yu, Seto, Wai-Kay, Fung, James, Lai, Ching-Lung, Yuen, Man-Fung
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2014
Sujets:
Virology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4313139/
https://ncbi.nlm.nih.gov/pubmed/24951803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00343-14
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