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Release of Escherichia coli DNA from membrane complexes by single-strand endonucleases.

Treatment of gently prepared lysates of Escherichia coli with single-strand-specific endonuclease (SI or from mung beans) results in the release of about 90% of the DNA from membranes, as determined by the M band technique. The released DNA has an average molecular weight of about 1.2 X 10(8). Data...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Abe, M, Brown, C, Hendrickson, W G, Boyd, D H, Clifford, P, Cote, R H, Schaechter, M
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1977
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431278/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/331316/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.7.2756
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