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Negative homotropic cooperativity and affinity heterogeneity: preparation of yeast glyceraldehyde-3-phosphate dehydrogenase with maximal affinity homogeneity.
A three-step procedure including affinity chromatography on NAD+-azobenzamidopropyl-Sepharose has been designed for the purification of yeast glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate: NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12] with maximized specific activity a...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Author: | |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1976
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431269/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/186779/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.73.11.3928 |
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