Biochemical construction of specific chimeric plasmids from ColE1 DNA and unfractionated Escherichia coli DNA.

A series of chimeric plasmids was constructed using colicinigenic factor E1 (ColE1) DNA as the replicon and DNA fragments carrying the galactose or tryptophan operons from E. coli. Restriction endonuclease EcoRI digests of ColE1 DNA and various DNAs containing the trp or gal operons were joined by T...

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Bibliographic Details
Main Authors: Collins, C J, Jackson, D A, DeVries, A J
Format: Artigo
Language:Inglês
Published: 1976
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC431231/
https://ncbi.nlm.nih.gov/pubmed/792875
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