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Restriction assay for integrative recombination of bacteriophage lambda DNA in vitro: requirement for closed circular DNA substrate.

A novel assay has been developed for in vitro genetic recombination of DNA. Substrate and product DNAs are cleaved with a restriction endonuclease and the resulting fragments are separated by electrophoresis in agarose gels. The substrate DNA has been chosen so that the recombination to be studied d...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Mizuuchi, K, Nash, H A
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1976
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431149/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1068464/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.73.10.3524
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