A general method for cloning eukaryotic structural gene sequences.

Complementary DNA, transcribed in vitro from purified rabbit globin messenger RNA and made double-stranded, has been inserted into Escherichia coli plasmids pSC101 and pMB9 by the poly(dT)/poly(dA) "tailing" and annealing technique. E. coli transformants given by this DNA preparation have been shown...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Higuchi, R, Paddock, G V, Wall, R, Salser, W
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1976
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC430960/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/61588/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.73.9.3146
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