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Endonuclease Specificity and Sequence Dependence of Type IIS Restriction Enzymes

Restriction enzymes that recognize specific sequences but cleave unknown sequence outside the recognition site are extensively utilized tools in molecular biology. Despite this, systematic functional categorization of cleavage performance has largely been lacking. We established a simple and automat...

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Bibliographische Detailangaben
Veröffentlicht in:PLoS One
Hauptverfasser: Lundin, Sverker, Jemt, Anders, Terje-Hegge, Finn, Foam, Napoleon, Pettersson, Erik, Käller, Max, Wirta, Valtteri, Lexow, Preben, Lundeberg, Joakim
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4309577/
https://ncbi.nlm.nih.gov/pubmed/25629514
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0117059
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